In many countries screening for Glucose-6-Phosphate Dehydrogenase (G6PD) is a part of thenewborn screening program. In Indonesia the National Newborn screening program started in 2012 exclu-sively for Congenital Hypothyroidism and screening for G6PD has not been included in the National Screen-ing Program. Deficiency of G6PD can cause many manifestations with high morbidity rate. Different areasin Indonesia might have different proportion of G6PD deficiency. In Indonesia, there is no report on screen-ing for G6PD deficiencies in newborn babies. Objective: To estimate the prevalence of G6PD deficiencies inIndonesian newborn. Methodology: Dried blood spot (DBS) samples were collected from many provinces allover Indonesia as part of the National Screening Program for Hypothyroid, and 676 DBS samples receivedin May–June 2017 were analyzed for G6PD deficiency using LabSystem Neonatal G6PD kit and FluorometerThermo Scientific instrument. Results: From 676 newborn this study it was found that the G6PD deficiency wasobserved in 7.39 male and 6.95% female babies. The cutoff value for G6PD deficiency was less than 4,5 IU/gHb.The proportion was higher in males babies compared to females. Based on this finding, the G6PD screeningshould be performed in all newborn because the total prevalence was more than 3–5% as stated by the WHOrecommendation