Newborn screening (NBS) for congenital hypothyroidism (CH) started in the 1970s, with the introduction of radioimmunoassays (RIA) for the measurement of thyroxine (T4), and thyroid stimulating hormone (TSH). With the development of sensitive enzyme immune assays (EIA, FIA, FEIA), RIAs were replaced in newborn screening laboratories. With the increasing number of analytes and centralization of NBS, there is a growing demand of total automation. In the course of method validation, two fully automated platforms for the determination of TSH in dried blood samples (DBS) were compared. The GSP from PerkinElmer (PE), and the NS2400 from Labsystems (LDx), together with the recommended test kits from both manufacturers. Both systems showed good performance, with recoveries, of 103.0% (LDx) and 98.5% (PE), and CVs for intra and interassay variations at various concentrations, between 4.3 and 15.7. Both assays had a good correlation (r2 = 0.8814). No false negative results were observed with either of the two platforms. In conclusion, both platforms are equally suitable for medium and large NBS laboratories. However, due to the more open structure, the LDx/NS2400 platform has a lot of advantages compared to the totally closed PE/GSP platform.